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Home?>?Products & Services?>?Nucleic Acid?>?Nucleic?>?PCR?>?2×熱啟動PCR預(yù)混液

2×熱啟動PCR預(yù)混液

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MonAmp? 2× HS Taq Mix的濃度為2×,使用方便快捷,能減少PCR操作過程中的污染,使用時只需取適量MonAmp? 2× HS Taq Mix,加入模板和引物,并加入ddH2O補足體積,使反應(yīng)體系濃度為1×即可進行PCR反應(yīng)。 Mix中包含熱啟動MonTaq-HS DNA Polymerase,在PCR反應(yīng)開始前,該酶的聚合酶活性受到抑制,防止低溫條件下非特異性退火或引物二聚體導(dǎo)致的非特異性擴增。該Mix具有良好的擴增特異性和模板兼容性,最長可擴增5 kb DNA片段,PCR產(chǎn)物3'端帶A堿基,純化后可直接用于T/A克隆。

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